The effect of Trichoderma Spp. on physiological parameters of two tomato cultivars grown under greenhouse conditions
Аутори
Bojović, MirjanaVukelić, Igor
Srećkov, Zorana
Mrkonjić, Zorica
Radić, Danka
Racić, Gordana
Panković, Dejana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
INTRODUCTION:
Fungi that belong to the genus Trichoderma colonize plant rhizosphere and are considered as good candidates for the use
in environmentally friendly agriculture as plant growth promoters and biocontrol agents. In recent years, investigations are
focused on the use of non-destructive characterization of plant responses to different abiotic and biotic stresses.
OBJECTIVES:
Dualex sensor was used in order to pre-screen the reaction of tomato plants treated with different Trichoderma isolates,
based on the previous investigations that imply that the positive effects of this fungi depend on the plant genotype.
METHOD / DESIGN:
The experiment was conducted in a randomized block design under greenhouse conditions with two replicates. In total 30
plants of two tomato cultivars were transplanted in soil, per treatment: NC-control Narvik, GZC- control Gruzanski zlatni,
NT1 - T. harzianum; NT2 - T. brevicompactum; NT3 - T. harzianum + T. brevicompactum; GZT1 - T. harzianum;... GZT2 - T. brevicompactum;
GZT3 - T. harzianum + T. brevicompactum). The suspensions of Trichoderma isolates were applied in the root zone
of tomato plants, in the phase of three established leaves per plant. Measurements of chlorophyll (Chl), flavonols (Flav) and
anthocyanins (Ant) content were done in vivo on fully developed leaves of the tomato plants, using Dualex optical sensor
(Force-A, Orsay, France), once per week during 50 days of plant growth. NBI (Nitrogen Balance Index) was calculated as Chl/
Flav ratio.
RESULTS:
Results obtained in this experiment showed that in both cultivars, the content of chlorophyll did not change significantly
after Trichoderma application. However, positive trend for Flav content was observed for the cultivar GZ in the treatment with
T2. In contrast, at the beginning of the experiment, NBI index decreased in GZ as influenced with T1 and T2 treatments, which
could indicate a shift from primary to secondary metabolism in mentioned cultivar. Moreover, it could be noticed that during
plant growth content of anthocyanin decreased in both control and Trichoderma treatment conditions.
CONCLUSIONS:
In conclusion, we can suggest that use of non-destructive measurements with Dualex sensor could serve as starting point
to better understanding of plant responses to Trichoderma presence. Moreover, measurements with Dualex can serve as
a pre-screening method for testing the effect of larger number of Trichoderma isolates and their effect on more different
tomato genotypes.
Кључне речи:
tomato / Trichoderma / greenhouse / non-destructive measurementsИзвор:
THE International Bioscience Conference (2021 ; Novi Sad) [Book of abstracts] / The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021, 25-26 November, 2021, 2021, 116-117Издавач:
- Novi Sad : Prirodno-matematički fakultet
Финансирање / пројекти:
- Provincial Secretary for Higher Education and Scientific Research, Autonomous Province of Vojvodina, No. (142-451-2419/2021)
Институција/група
Fakultet ekološke poljoprivredeTY - CONF AU - Bojović, Mirjana AU - Vukelić, Igor AU - Srećkov, Zorana AU - Mrkonjić, Zorica AU - Radić, Danka AU - Racić, Gordana AU - Panković, Dejana PY - 2021 UR - https://redun.educons.edu.rs/handle/123456789/543 AB - INTRODUCTION: Fungi that belong to the genus Trichoderma colonize plant rhizosphere and are considered as good candidates for the use in environmentally friendly agriculture as plant growth promoters and biocontrol agents. In recent years, investigations are focused on the use of non-destructive characterization of plant responses to different abiotic and biotic stresses. OBJECTIVES: Dualex sensor was used in order to pre-screen the reaction of tomato plants treated with different Trichoderma isolates, based on the previous investigations that imply that the positive effects of this fungi depend on the plant genotype. METHOD / DESIGN: The experiment was conducted in a randomized block design under greenhouse conditions with two replicates. In total 30 plants of two tomato cultivars were transplanted in soil, per treatment: NC-control Narvik, GZC- control Gruzanski zlatni, NT1 - T. harzianum; NT2 - T. brevicompactum; NT3 - T. harzianum + T. brevicompactum; GZT1 - T. harzianum; GZT2 - T. brevicompactum; GZT3 - T. harzianum + T. brevicompactum). The suspensions of Trichoderma isolates were applied in the root zone of tomato plants, in the phase of three established leaves per plant. Measurements of chlorophyll (Chl), flavonols (Flav) and anthocyanins (Ant) content were done in vivo on fully developed leaves of the tomato plants, using Dualex optical sensor (Force-A, Orsay, France), once per week during 50 days of plant growth. NBI (Nitrogen Balance Index) was calculated as Chl/ Flav ratio. RESULTS: Results obtained in this experiment showed that in both cultivars, the content of chlorophyll did not change significantly after Trichoderma application. However, positive trend for Flav content was observed for the cultivar GZ in the treatment with T2. In contrast, at the beginning of the experiment, NBI index decreased in GZ as influenced with T1 and T2 treatments, which could indicate a shift from primary to secondary metabolism in mentioned cultivar. Moreover, it could be noticed that during plant growth content of anthocyanin decreased in both control and Trichoderma treatment conditions. CONCLUSIONS: In conclusion, we can suggest that use of non-destructive measurements with Dualex sensor could serve as starting point to better understanding of plant responses to Trichoderma presence. Moreover, measurements with Dualex can serve as a pre-screening method for testing the effect of larger number of Trichoderma isolates and their effect on more different tomato genotypes. PB - Novi Sad : Prirodno-matematički fakultet C3 - THE International Bioscience Conference (2021 ; Novi Sad) [Book of abstracts] / The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021, 25-26 November, 2021 T1 - The effect of Trichoderma Spp. on physiological parameters of two tomato cultivars grown under greenhouse conditions EP - 117 SP - 116 ER -
@conference{ author = "Bojović, Mirjana and Vukelić, Igor and Srećkov, Zorana and Mrkonjić, Zorica and Radić, Danka and Racić, Gordana and Panković, Dejana", year = "2021", abstract = "INTRODUCTION: Fungi that belong to the genus Trichoderma colonize plant rhizosphere and are considered as good candidates for the use in environmentally friendly agriculture as plant growth promoters and biocontrol agents. In recent years, investigations are focused on the use of non-destructive characterization of plant responses to different abiotic and biotic stresses. OBJECTIVES: Dualex sensor was used in order to pre-screen the reaction of tomato plants treated with different Trichoderma isolates, based on the previous investigations that imply that the positive effects of this fungi depend on the plant genotype. METHOD / DESIGN: The experiment was conducted in a randomized block design under greenhouse conditions with two replicates. In total 30 plants of two tomato cultivars were transplanted in soil, per treatment: NC-control Narvik, GZC- control Gruzanski zlatni, NT1 - T. harzianum; NT2 - T. brevicompactum; NT3 - T. harzianum + T. brevicompactum; GZT1 - T. harzianum; GZT2 - T. brevicompactum; GZT3 - T. harzianum + T. brevicompactum). The suspensions of Trichoderma isolates were applied in the root zone of tomato plants, in the phase of three established leaves per plant. Measurements of chlorophyll (Chl), flavonols (Flav) and anthocyanins (Ant) content were done in vivo on fully developed leaves of the tomato plants, using Dualex optical sensor (Force-A, Orsay, France), once per week during 50 days of plant growth. NBI (Nitrogen Balance Index) was calculated as Chl/ Flav ratio. RESULTS: Results obtained in this experiment showed that in both cultivars, the content of chlorophyll did not change significantly after Trichoderma application. However, positive trend for Flav content was observed for the cultivar GZ in the treatment with T2. In contrast, at the beginning of the experiment, NBI index decreased in GZ as influenced with T1 and T2 treatments, which could indicate a shift from primary to secondary metabolism in mentioned cultivar. Moreover, it could be noticed that during plant growth content of anthocyanin decreased in both control and Trichoderma treatment conditions. CONCLUSIONS: In conclusion, we can suggest that use of non-destructive measurements with Dualex sensor could serve as starting point to better understanding of plant responses to Trichoderma presence. Moreover, measurements with Dualex can serve as a pre-screening method for testing the effect of larger number of Trichoderma isolates and their effect on more different tomato genotypes.", publisher = "Novi Sad : Prirodno-matematički fakultet", journal = "THE International Bioscience Conference (2021 ; Novi Sad) [Book of abstracts] / The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021, 25-26 November, 2021", title = "The effect of Trichoderma Spp. on physiological parameters of two tomato cultivars grown under greenhouse conditions", pages = "117-116" }
Bojović, M., Vukelić, I., Srećkov, Z., Mrkonjić, Z., Radić, D., Racić, G.,& Panković, D.. (2021). The effect of Trichoderma Spp. on physiological parameters of two tomato cultivars grown under greenhouse conditions. in THE International Bioscience Conference (2021 ; Novi Sad) [Book of abstracts] / The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021, 25-26 November, 2021 Novi Sad : Prirodno-matematički fakultet., 116-117.
Bojović M, Vukelić I, Srećkov Z, Mrkonjić Z, Radić D, Racić G, Panković D. The effect of Trichoderma Spp. on physiological parameters of two tomato cultivars grown under greenhouse conditions. in THE International Bioscience Conference (2021 ; Novi Sad) [Book of abstracts] / The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021, 25-26 November, 2021. 2021;:116-117..
Bojović, Mirjana, Vukelić, Igor, Srećkov, Zorana, Mrkonjić, Zorica, Radić, Danka, Racić, Gordana, Panković, Dejana, "The effect of Trichoderma Spp. on physiological parameters of two tomato cultivars grown under greenhouse conditions" in THE International Bioscience Conference (2021 ; Novi Sad) [Book of abstracts] / The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021, 25-26 November, 2021 (2021):116-117.