Potential of Helicrysum italicum cultivated in urban environment: SCCO2 extract cytotoxicity & NF-kB activation in HeLa, MCF-7 and MRC-5 cells
Nema prikaza
Autori
Jovanović-Galović, AleksandraJovanović-Ljesković, Natasa
Vidović, Senka
Vladić, Jelena
Mrkonjić, Zorica
Gigov, Slobodan
Ilić, Milan
Kojić, Vesna
Jakimov, Dimitar
Zloh, Mire
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Urban horticulture and vertical gardens provide many benefits for the population in urban areas. A rational selection of plants in these features can introduce a potential for value-added products including extracts of nutraceuticals. Helichrysum. italicum was grown in the urban environment, on a vertical surface in the form of a green wall. Since the location was in a town with continental climate, particular attention was given to ensure that the minimum humidity of the soil and maximal insolation were achieved. Supercritical CO2 (SCCO2) was used for extraction of phytochemicals. Arzanol content of the obtained extracts was determined by HPLC-DAD using pure arzanol as standard. Cytotoxicity (MTT test) and NF-kB activation (p65 ELISA) were evaluated upon treatment of cells in culture (MCF-7, HeLa, MRC-5) with the SCCO2 extracts. This study demonstrates that H. italicum cultivation in urban conditions in the continental climate yields phytochemical-rich extracts. Obtained plant extract...s showed potential anticancer effects when investigated in cell-based assays. Extract concentrations below IC50 concentrations for cytotoxic effects had a significant effect on NF-kB activation that was cell-line specific. HeLa and MCF-7 cells responded differently - suggesting the importance of cell receptors for biological activity. Further analysis of the potential use of extracts should be conducted using animal models. The reported use of Helicrysum italicum in the green healing wall is in line with the UN Development Goals for sustainable cities to improve the urban environment with benefits for the population wellbeing and a potential to obtain value-added bioactive extracts.
Ključne reči:
SCCO2 extraction / Medicinal plant / Helichrysum italicum / Green wall / ArzanolIzvor:
Sustainable Chemistry and Pharmacy, 2022, 26Izdavač:
- ELSEVIER, AMSTERDAM
Finansiranje / projekti:
- City of Novi Sad - Administration for Environmental Protection, Novi Sad, Serbia [VI-501-2/2018-18?-19]
Institucija/grupa
Fakultet ekološke poljoprivredeTY - JOUR AU - Jovanović-Galović, Aleksandra AU - Jovanović-Ljesković, Natasa AU - Vidović, Senka AU - Vladić, Jelena AU - Mrkonjić, Zorica AU - Gigov, Slobodan AU - Ilić, Milan AU - Kojić, Vesna AU - Jakimov, Dimitar AU - Zloh, Mire PY - 2022 UR - https://redun.educons.edu.rs/handle/123456789/467 AB - Urban horticulture and vertical gardens provide many benefits for the population in urban areas. A rational selection of plants in these features can introduce a potential for value-added products including extracts of nutraceuticals. Helichrysum. italicum was grown in the urban environment, on a vertical surface in the form of a green wall. Since the location was in a town with continental climate, particular attention was given to ensure that the minimum humidity of the soil and maximal insolation were achieved. Supercritical CO2 (SCCO2) was used for extraction of phytochemicals. Arzanol content of the obtained extracts was determined by HPLC-DAD using pure arzanol as standard. Cytotoxicity (MTT test) and NF-kB activation (p65 ELISA) were evaluated upon treatment of cells in culture (MCF-7, HeLa, MRC-5) with the SCCO2 extracts. This study demonstrates that H. italicum cultivation in urban conditions in the continental climate yields phytochemical-rich extracts. Obtained plant extracts showed potential anticancer effects when investigated in cell-based assays. Extract concentrations below IC50 concentrations for cytotoxic effects had a significant effect on NF-kB activation that was cell-line specific. HeLa and MCF-7 cells responded differently - suggesting the importance of cell receptors for biological activity. Further analysis of the potential use of extracts should be conducted using animal models. The reported use of Helicrysum italicum in the green healing wall is in line with the UN Development Goals for sustainable cities to improve the urban environment with benefits for the population wellbeing and a potential to obtain value-added bioactive extracts. PB - ELSEVIER, AMSTERDAM T2 - Sustainable Chemistry and Pharmacy T1 - Potential of Helicrysum italicum cultivated in urban environment: SCCO2 extract cytotoxicity & NF-kB activation in HeLa, MCF-7 and MRC-5 cells VL - 26 DO - 10.1016/j.scp.2022.100622 UR - conv_1121 ER -
@article{ author = "Jovanović-Galović, Aleksandra and Jovanović-Ljesković, Natasa and Vidović, Senka and Vladić, Jelena and Mrkonjić, Zorica and Gigov, Slobodan and Ilić, Milan and Kojić, Vesna and Jakimov, Dimitar and Zloh, Mire", year = "2022", abstract = "Urban horticulture and vertical gardens provide many benefits for the population in urban areas. A rational selection of plants in these features can introduce a potential for value-added products including extracts of nutraceuticals. Helichrysum. italicum was grown in the urban environment, on a vertical surface in the form of a green wall. Since the location was in a town with continental climate, particular attention was given to ensure that the minimum humidity of the soil and maximal insolation were achieved. Supercritical CO2 (SCCO2) was used for extraction of phytochemicals. Arzanol content of the obtained extracts was determined by HPLC-DAD using pure arzanol as standard. Cytotoxicity (MTT test) and NF-kB activation (p65 ELISA) were evaluated upon treatment of cells in culture (MCF-7, HeLa, MRC-5) with the SCCO2 extracts. This study demonstrates that H. italicum cultivation in urban conditions in the continental climate yields phytochemical-rich extracts. Obtained plant extracts showed potential anticancer effects when investigated in cell-based assays. Extract concentrations below IC50 concentrations for cytotoxic effects had a significant effect on NF-kB activation that was cell-line specific. HeLa and MCF-7 cells responded differently - suggesting the importance of cell receptors for biological activity. Further analysis of the potential use of extracts should be conducted using animal models. The reported use of Helicrysum italicum in the green healing wall is in line with the UN Development Goals for sustainable cities to improve the urban environment with benefits for the population wellbeing and a potential to obtain value-added bioactive extracts.", publisher = "ELSEVIER, AMSTERDAM", journal = "Sustainable Chemistry and Pharmacy", title = "Potential of Helicrysum italicum cultivated in urban environment: SCCO2 extract cytotoxicity & NF-kB activation in HeLa, MCF-7 and MRC-5 cells", volume = "26", doi = "10.1016/j.scp.2022.100622", url = "conv_1121" }
Jovanović-Galović, A., Jovanović-Ljesković, N., Vidović, S., Vladić, J., Mrkonjić, Z., Gigov, S., Ilić, M., Kojić, V., Jakimov, D.,& Zloh, M.. (2022). Potential of Helicrysum italicum cultivated in urban environment: SCCO2 extract cytotoxicity & NF-kB activation in HeLa, MCF-7 and MRC-5 cells. in Sustainable Chemistry and Pharmacy ELSEVIER, AMSTERDAM., 26. https://doi.org/10.1016/j.scp.2022.100622 conv_1121
Jovanović-Galović A, Jovanović-Ljesković N, Vidović S, Vladić J, Mrkonjić Z, Gigov S, Ilić M, Kojić V, Jakimov D, Zloh M. Potential of Helicrysum italicum cultivated in urban environment: SCCO2 extract cytotoxicity & NF-kB activation in HeLa, MCF-7 and MRC-5 cells. in Sustainable Chemistry and Pharmacy. 2022;26. doi:10.1016/j.scp.2022.100622 conv_1121 .
Jovanović-Galović, Aleksandra, Jovanović-Ljesković, Natasa, Vidović, Senka, Vladić, Jelena, Mrkonjić, Zorica, Gigov, Slobodan, Ilić, Milan, Kojić, Vesna, Jakimov, Dimitar, Zloh, Mire, "Potential of Helicrysum italicum cultivated in urban environment: SCCO2 extract cytotoxicity & NF-kB activation in HeLa, MCF-7 and MRC-5 cells" in Sustainable Chemistry and Pharmacy, 26 (2022), https://doi.org/10.1016/j.scp.2022.100622 ., conv_1121 .