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dc.creatorPanković, Dejana
dc.creatorRadovanović, N.
dc.creatorJocić, S.
dc.creatorSatović, Zlatko
dc.creatorSkorić, D.
dc.date.accessioned2023-04-07T13:41:06Z
dc.date.available2023-04-07T13:41:06Z
dc.date.issued2007
dc.identifier.issn0179-9541
dc.identifier.urihttps://redun.educons.edu.rs/handle/123456789/35
dc.description.abstractThe inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F-3 families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F-3 families were inoculated, among which 54 families were resistant and 19 susceptible, fitting a 3 : 1 segregation ratio. F-3 families were also studied using several PCR markers. Ten markers at the Pl6 locus, specific for the resistant line, also segregated in F-3 families with a 3 : 1 ratio. The same segregation ratio occurred for microsatellite haplotypes that resembled the resistant parent, and were amplified with ORS 166 and ORS 1043. The only common fragment that was observed between resistant and susceptible parental lines was one of the TIR-NBS-LRR resistance gene analogue markers, having a restriction site. Two co-dominant cleaved amplified polymorphic sequence (CAPS) markers were obtained. The mapping data indicate that several dominant markers and two CAPS markers, developed here, completely co-segregate with the Pl6 gene conferring resistance to race 730. CAPS markers will facilitate efficient marker-assisted selection for sunflower resistance to downy mildew race 730.en
dc.publisherWILEY, HOBOKEN
dc.rightsrestrictedAccess
dc.sourcePlant Breeding
dc.subjectPlasmopara halstediien
dc.subjectmarker-assisted selectionen
dc.subjectHelianthus annuusen
dc.subjectcleaved amplified polymorphic sequencesen
dc.titleDevelopment of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730en
dc.typearticle
dc.rights.licenseARR
dc.citation.epage444
dc.citation.issue4
dc.citation.other126(4): 440-444
dc.citation.rankM22
dc.citation.spage440
dc.citation.volume126
dc.identifier.doi10.1111/j.1439-0523.2007.01376.x
dc.identifier.rcubconv_697
dc.identifier.scopus2-s2.0-34447508012
dc.identifier.wos000248849400018
dc.type.versionpublishedVersion


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