Species composition of trichoderma isolates from the rhizosphere of vegetables grown in Hungarian soils
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Koermoeczi, PeterRacić, Gordana
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Manczinger, Laszlo
Jovanović, Ljubinko
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Panković, Dejana
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Vagvoelgyi, Csaba
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Kredics, Laszlo
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The species composition of Trichoderma isolates from the rhizosphere of different vegetables collected at different locations in Hungary was examined during this study. Trichoderma strains were isolated from the rhizosphere samples on dichloran-rose bengal medium. After purification of genomic DNA, the PCR amplification of the internal transcribed spacer (ITS1-5.8S rDNA-ITS2) region and its sequence analysis were used for the identification of the isolates at the species level. Altogether, 45 Trichoderma isolates were identified from the examined samples. The detected Trichoderma species were T. asperellum, T. atroviride, T. citrinoviride, T. gamsii, T. hamatum, T. harzianum, T. koningiopsis/T ovalisporum, T. longibrachiatum/H. orientalis, T. pleuroticola and T. virens. Besides species known as opportunistic pathogens of humans (T. longibrachiatum / H. orientalis, T. citrinoviride) or as causative agents of mushroom green mould disease (T. pleuroticola), beneficial taxa (T. harzianum, ...T virens, T. atroviride) widely used for the biological control of plant pathogenic fungi could also be identified in the examined samples, suggesting that the rhizosphere of vegetables may be a rich source of potential biocontrol agents. In vitro antagonism was examined in dual culture tests and the Biocontrol Index (BCI) values were determined for the particular isolates. Certain T. asperellum, T. virens and T. atroviride isolates proved to possess good in vitro antagonistic activities against plant pathogenic Fusarium solani and F. oxysporum strains, suggesting that they might be promising for the development of Trichoderma-based biocontrol strategies for the suppression of plant pathogenic fungi in the rhizosphere of vegetables produced in organic farmland soils.
Keywords:
vegetables / Trichoderma / species composition / rhizosphere / biocontrolSource:
Fresenius Environmental Bulletin, 2013, 22, 6, 1736-1741Funding / projects:
- European Union through the Hungary-Serbia IPA Cross-border Co-operation Programme (PHANETRI) [HUSRB/1002/214/068]
- National Excellence Program - Elaborating and operating an inland student and researcher personal support system [TAMOP 4.2.4. A/2-11-1-2012-0001]
- European Union
- European Social Fund
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Fakultet ekološke poljoprivredeTY - JOUR AU - Koermoeczi, Peter AU - Racić, Gordana AU - Manczinger, Laszlo AU - Jovanović, Ljubinko AU - Panković, Dejana AU - Vagvoelgyi, Csaba AU - Kredics, Laszlo PY - 2013 UR - https://redun.educons.edu.rs/handle/123456789/179 AB - The species composition of Trichoderma isolates from the rhizosphere of different vegetables collected at different locations in Hungary was examined during this study. Trichoderma strains were isolated from the rhizosphere samples on dichloran-rose bengal medium. After purification of genomic DNA, the PCR amplification of the internal transcribed spacer (ITS1-5.8S rDNA-ITS2) region and its sequence analysis were used for the identification of the isolates at the species level. Altogether, 45 Trichoderma isolates were identified from the examined samples. The detected Trichoderma species were T. asperellum, T. atroviride, T. citrinoviride, T. gamsii, T. hamatum, T. harzianum, T. koningiopsis/T ovalisporum, T. longibrachiatum/H. orientalis, T. pleuroticola and T. virens. Besides species known as opportunistic pathogens of humans (T. longibrachiatum / H. orientalis, T. citrinoviride) or as causative agents of mushroom green mould disease (T. pleuroticola), beneficial taxa (T. harzianum, T virens, T. atroviride) widely used for the biological control of plant pathogenic fungi could also be identified in the examined samples, suggesting that the rhizosphere of vegetables may be a rich source of potential biocontrol agents. In vitro antagonism was examined in dual culture tests and the Biocontrol Index (BCI) values were determined for the particular isolates. Certain T. asperellum, T. virens and T. atroviride isolates proved to possess good in vitro antagonistic activities against plant pathogenic Fusarium solani and F. oxysporum strains, suggesting that they might be promising for the development of Trichoderma-based biocontrol strategies for the suppression of plant pathogenic fungi in the rhizosphere of vegetables produced in organic farmland soils. T2 - Fresenius Environmental Bulletin T1 - Species composition of trichoderma isolates from the rhizosphere of vegetables grown in Hungarian soils EP - 1741 IS - 6 SP - 1736 VL - 22 UR - conv_1192 ER -
@article{ author = "Koermoeczi, Peter and Racić, Gordana and Manczinger, Laszlo and Jovanović, Ljubinko and Panković, Dejana and Vagvoelgyi, Csaba and Kredics, Laszlo", year = "2013", abstract = "The species composition of Trichoderma isolates from the rhizosphere of different vegetables collected at different locations in Hungary was examined during this study. Trichoderma strains were isolated from the rhizosphere samples on dichloran-rose bengal medium. After purification of genomic DNA, the PCR amplification of the internal transcribed spacer (ITS1-5.8S rDNA-ITS2) region and its sequence analysis were used for the identification of the isolates at the species level. Altogether, 45 Trichoderma isolates were identified from the examined samples. The detected Trichoderma species were T. asperellum, T. atroviride, T. citrinoviride, T. gamsii, T. hamatum, T. harzianum, T. koningiopsis/T ovalisporum, T. longibrachiatum/H. orientalis, T. pleuroticola and T. virens. Besides species known as opportunistic pathogens of humans (T. longibrachiatum / H. orientalis, T. citrinoviride) or as causative agents of mushroom green mould disease (T. pleuroticola), beneficial taxa (T. harzianum, T virens, T. atroviride) widely used for the biological control of plant pathogenic fungi could also be identified in the examined samples, suggesting that the rhizosphere of vegetables may be a rich source of potential biocontrol agents. In vitro antagonism was examined in dual culture tests and the Biocontrol Index (BCI) values were determined for the particular isolates. Certain T. asperellum, T. virens and T. atroviride isolates proved to possess good in vitro antagonistic activities against plant pathogenic Fusarium solani and F. oxysporum strains, suggesting that they might be promising for the development of Trichoderma-based biocontrol strategies for the suppression of plant pathogenic fungi in the rhizosphere of vegetables produced in organic farmland soils.", journal = "Fresenius Environmental Bulletin", title = "Species composition of trichoderma isolates from the rhizosphere of vegetables grown in Hungarian soils", pages = "1741-1736", number = "6", volume = "22", url = "conv_1192" }
Koermoeczi, P., Racić, G., Manczinger, L., Jovanović, L., Panković, D., Vagvoelgyi, C.,& Kredics, L.. (2013). Species composition of trichoderma isolates from the rhizosphere of vegetables grown in Hungarian soils. in Fresenius Environmental Bulletin, 22(6), 1736-1741. conv_1192
Koermoeczi P, Racić G, Manczinger L, Jovanović L, Panković D, Vagvoelgyi C, Kredics L. Species composition of trichoderma isolates from the rhizosphere of vegetables grown in Hungarian soils. in Fresenius Environmental Bulletin. 2013;22(6):1736-1741. conv_1192 .
Koermoeczi, Peter, Racić, Gordana, Manczinger, Laszlo, Jovanović, Ljubinko, Panković, Dejana, Vagvoelgyi, Csaba, Kredics, Laszlo, "Species composition of trichoderma isolates from the rhizosphere of vegetables grown in Hungarian soils" in Fresenius Environmental Bulletin, 22, no. 6 (2013):1736-1741, conv_1192 .